Diagnostics Automation


A50-Universal Flow Cytometer

A50-Universal Flow Cytometer


  • High speed - up to 100k events/sec. (requires optional "full-digital" electronics)
  • Volumetric sample system gives absolute count (avoids expensive, problematic counting beads)
  • Built in PC with Windows XP & remote assistance option
  • Up to nine fluorescence detectors
  • One or two light scatter detectors
  • A50 model offers up to 3 lasers


  • Immunology (e.g. lymphocyte phenotyping)
  • Haematology (e.g. platelet analysis)
  • DNA analysis
  • Plant ploidy analysis
  • Apoptosis & viability
  • ...and more

Technical Data

Installation Requirements:


W32 x H48 x D48cm


Approx. 25kg


100-240VAC, 50/60Hz


No special requirements

Operating Environment

5 to 35 degrees Centigrade


Numerical Aperture



A50-Universal: Up to three lasers: 375nm, 405nm, 488nm, 532nm, 635nm or almost any other commercially available wavelength.

Optical Filter Blocks

Interchangeable without realignment

Light Scatter Optics

One or two scatter detectors
Sensitivity: 300nm (latex beads)

Fluorescence Optics

Up to 9 colours (channels) with interchangeable filter blocks

Optical Detectors

Photomultiplier tubes on all channels



Volumetric sample injection
Adjustable sample aspiration volume, 100-300µl (smaller and larger volumes available on request)
Selectable, precise sample flow rate from 1.4 to 100ul/min to suit a wide range of sample dilutions (adjustable during sample acquisition)
Optional automatic sampling from 96 well plates or microcentrifuge tubes


Choice of Closed loop ultra-pure sheath fluid system or refillable sheath tank with level sensor

Sample Range

Sample concentrations up to 108 per ml

Data Management:


Fast electronics: Up to 100,000 events per second (requires new 'full-digital' electronic option)
Threshold software selectable on all channels with 'AND' or 'OR' logic
Pulse height and pulse area measurements on all channels
Event time stamp for each particle
18 bit data acquisition (5 logarithmic decades)
Internal PC with Windows XP operating system
Optional external desktop or laptop PC with LAN connection.


Histogram resolution software selectable from 32 to 65535
Display modes: linear and/or logarithmic gain selection for each parameter
Unlimited number of histograms and cytograms
Amorphous regions of interest (gates)
Gating using AND, OR and NOT logic
RATIO parameter configurable for any 2 detectors
Autocycle module
Gain and subtraction settings adjustable during and after acquisition
FCS 2.0 compatible files storage, and storage in 11 or 16 bit format
Export of statistics from multiple files to Microsoft Excel
Export of histogram data in '.csv' format
Quick copy to clipboard feature for datagrams
Overlaying histograms for easy data comparison.
Plus many more features.

Data Analysis Automation

Optional Auto-Lymphocyte cluster analysis and gating software

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